Western blot analysis of extracts of various cell lines, using Cyclin H antibody (CAB21885) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The Cyclin H Monoclonal Antibody (CAB21885) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed by Assay Genie, specifically targets cyclin H, a key protein involved in cell cycle progression and DNA repair processes.Cyclin H is known to form complexes with CDK7 and MAT1, contributing to the activation of cyclin-dependent kinases (CDKs) and ultimately regulating cell cycle checkpoints. Dysregulation of cyclin H has been linked to various cancer types, making it a promising target for therapeutic intervention.This monoclonal antibody is highly specific and sensitive, allowing for accurate detection and analysis of cyclin H levels in various cell types and tissues.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Cyclin H Monoclonal Antibody
SKU:
CAB21885
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC2823
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CAK, p34, p37, CycH, Cyclin H
Positive Sample:
NIH/3T3, Jurkat, Mouse testis
Cellular Localization:
Nucleus.
Calculated MW:
38kDa
Observed MW:
35kDa
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery. A pseudogene of this gene is found on chromosome 4. Alternate splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
902
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Cyclin H Rabbit mAb (CAB21885) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
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