The Cyclin E1 Rabbit Monoclonal Antibody (CAB22476) is a valuable tool for researchers studying the cell cycle and cancer biology. Cyclin E1 is a key regulator of cell cycle progression, particularly in the G1/S phase transition. This antibody, produced using rabbit monoclonal technology, is highly specific to Cyclin E1 and suitable for various experimental applications, including Western blot, immunofluorescence, and immunohistochemistry.Cyclin E1 is known to be overexpressed in various cancer types, where it promotes uncontrolled cell proliferation and tumorigenesis. By targeting Cyclin E1 with this antibody, researchers can investigate its role in cancer development and potentially identify novel therapeutic targets.
Additionally, studying Cyclin E1 expression and localization in different cell types can provide insights into its normal physiological functions and its dysregulation in disease states.Overall, the CAB22476 antibody offers a reliable and specific tool for researchers interested in exploring the mechanisms of cell cycle regulation and the implications of Cyclin E1 dysregulation in cancer. Its high reactivity and specificity make it a valuable asset for studies aiming to advance our understanding of cancer biology and potentially contribute to the development of novel anti-cancer therapies.
Product Name:
Cyclin E1 Rabbit Monoclonal Antibody
SKU:
CAB22476
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 300-400 of human Cyclin E1 (NP_001229.1).
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB.
Purification Method:
Affinity purification
Gene ID:
898
Clone Number:
ARC51384_ARC51385
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Cyclin E1 Rabbit PolymAb® (CAB22476) at 1:10000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
