The Cyclin E1 Monoclonal Antibody is a vital tool for researchers studying the cell cycle and cancer biology. This antibody specifically targets Cyclin E1, a key regulator of the cell cycle that is often dysregulated in cancer cells. The antibody, produced through monoclonal technology, exhibits high specificity and sensitivity towards Cyclin E1 in human samples, making it ideal for use in various applications such as Western blotting and immunohistochemistry.Cyclin E1 is a crucial protein involved in the control of cell division and proliferation, making it a potential target for cancer therapy.
Dysregulation of Cyclin E1 expression has been linked to various types of cancer, highlighting its importance in oncology research. By using the Cyclin E1 Monoclonal Antibody, researchers can gain valuable insights into the role of this protein in cancer development and progression, ultimately aiding in the development of targeted therapies for cancer treatment.
Product Name:
Cyclin E1 Monoclonal Antibody
SKU:
CAB22360
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 300-400 of human Cyclin E1 (NP_001229.1).
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB.
Purification Method:
Affinity purification
Gene ID:
898
Clone Number:
ARC51383
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse spleen, using Cyclin E1 Rabbit mAb (CAB22360) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
