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Cyclin B1 Rabbit Polyclonal Antibody (CAB16800)

SKU:
CAB16800
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Cyclin B1 Rabbit Polyclonal Antibody
SKU:CAB16800
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 350-433 of human CCNB1 (NP_114172.1).
Sequence:CLAL KILD NGEW TPTL QHYL SYTE ESLL PVMQ HLAK NVVM VNQG LTKH MTVK NKYA TSKH AKIS TLPQ LNSA LVQD LAKA VAKV
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200
Synonyms:CCNB; B1
Positive Sample:HeLa
Cellular Localization:Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center.
Calculated MW:48kDa
Observed MW:55kDa

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). The encoded protein is necessary for proper control of the G2/M transition phase of the cell cycle.

Purification Method:Affinity purification
Gene ID:891
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and Cyclin B1 knockout (KO) HeLa cells using [KO Validated] Cyclin B1 Rabbit pAb (CAB16800) at 1:1000 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.

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