The Cyclin B1 Polyclonal Antibody (CAB16800) is a valuable tool for researchers studying cell cycle regulation and cancer biology. This antibody, generated in rabbits, targets Cyclin B1, a key regulatory protein involved in cell division and progression through the cell cycle. Cyclin B1 is essential for the transition from G2 phase to mitosis, where it forms complexes with cyclin-dependent kinases to orchestrate the events leading to cell division. Dysregulation of Cyclin B1 expression has been implicated in various types of cancer, making it a promising target for therapeutic interventions.This antibody is highly specific and sensitive, allowing for the detection and analysis of Cyclin B1 in various cell types and experimental conditions.
It has been validated for use in Western blot applications, providing researchers with a reliable tool for investigating the role of Cyclin B1 in cell proliferation, differentiation, and tumorigenesis.By studying Cyclin B1 with this antibody, researchers can gain insights into the mechanisms underlying abnormal cell growth and division, ultimately advancing our understanding of cancer development and potentially identifying new therapeutic targets for cancer treatment.
Product Name:
Cyclin B1 Rabbit Polyclonal Antibody
SKU:
CAB16800
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 350-433 of human CCNB1 (NP_114172.1).
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). The encoded protein is necessary for proper control of the G2/M transition phase of the cell cycle.
Purification Method:
Affinity purification
Gene ID:
891
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and Cyclin B1 knockout (KO) HeLa cells using [KO Validated] Cyclin B1 Rabbit pAb (CAB16800) at 1:1000 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
