The CYBB Polyclonal Antibody (CAB12430) is a valuable tool for research involving CYBB, a gene encoding the cytochrome b-245 beta chain, a component of the phagocyte oxidase (PHOX) enzyme complex. This enzyme plays a crucial role in the respiratory burst process of phagocytic cells, which is essential for the destruction of ingested pathogens.The antibody, raised in rabbits, is highly specific and reactive with human samples, making it an ideal choice for studies in immunology and infectious diseases. It has been validated for use in various applications, including Western blotting, immunohistochemistry, and immunofluorescence, enabling the detection and analysis of CYBB expression in different cell types and tissues.
Understanding the role of CYBB in the immune response is essential for advancing our knowledge of host defense mechanisms and developing targeted therapies for infectious diseases and other pathologies related to immune dysfunction. The CYBB Polyclonal Antibody is a reliable tool for researchers studying the function and regulation of this important gene in health and disease.
Product Name:
NOX2/gp91phox Rabbit Polyclonal Antibody
SKU:
CAB12430
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 101-200 of human NOX2/gp91phox (NP_000388.2).
Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole.
Purification Method:
Affinity purification
Gene ID:
1536
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of RAW 264.7, using NOX2/gp91phox Rabbit pAb (CAB12430) at 1:900 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
