The IL8 Polyclonal Antibody (CAB12452) is a valuable tool for researchers studying the IL8 protein, a key mediator of inflammation and immune response. This antibody, produced in rabbits, is highly specific and reactive with human samples, making it ideal for use in Western blot applications. By binding to IL8, the antibody allows for precise detection and analysis of this important cytokine in various cell types.IL8, also known as CXCL8, plays a crucial role in recruiting immune cells to sites of infection or injury, as well as promoting angiogenesis and tumor growth.
Its dysregulation has been implicated in various inflammatory conditions and cancer progression. By studying IL8 activity and expression levels, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets.Overall, the IL8 Polyclonal Antibody is a reliable tool for researchers interested in studying the role of IL8 in inflammation, cancer, and other disease states. Its high specificity and validated performance make it a valuable asset for immunology and oncology research projects.
Product Name:
IL8 Rabbit Polyclonal Antibody
SKU:
CAB12452
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-99 of human IL8 (NP_000575.1).
The protein encoded by this gene is a member of the CXC chemokine family and is a major mediator of the inflammatory response. The encoded protein is commonly referred to as interleukin-8 (IL-8). IL-8 is secreted by mononuclear macrophages, neutrophils, eosinophils, T lymphocytes, epithelial cells, and fibroblasts. It functions as a chemotactic factor by guiding the neutrophils to the site of infection. Bacterial and viral products rapidly induce IL-8 expression. IL-8 also participates with other cytokines in the proinflammatory signaling cascade and plays a role in systemic inflammatory response syndrome (SIRS). This gene is believed to play a role in the pathogenesis of the lower respiratory tract infection bronchiolitis, a common respiratory tract disease caused by the respiratory syncytial virus (RSV). The overproduction of this proinflammatory protein is thought to cause the lung inflammation associated with csytic fibrosis. This proinflammatory protein is also suspected of playing a role in coronary artery disease and endothelial dysfunction. This protein is also secreted by tumor cells and promotes tumor migration, invasion, angiogenesis and metastasis. This chemokine is also a potent angiogenic factor. The binding of IL-8 to one of its receptors (IL-8RB/CXCR2) increases the permeability of blood vessels and increasing levels of IL-8 are positively correlated with increased severity of multiple disease outcomes (eg, sepsis). This gene and other members of the CXC chemokine gene family form a gene cluster in a region of chromosome 4q.
Purification Method:
Affinity purification
Gene ID:
3576
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of Recombinant Human IL-8 Protein using IL8 Rabbit pAb (CAB12452) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 10ng/5ng/1ng per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
