Anti-CUX1 Antibody (PACO62255)

Antibody Name:	CUX1 Antibody (PACO62255)
Antibody SKU:	PACO62255
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human Homeobox protein cut-like 1 protein (24-94AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

Immunofluorescence staining of Hela cells with PACO62255 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Background:

Probably has a broad role in mammalian development as a repressor of developmentally regulated gene expression. May act by preventing binding of positively-activing CCAAT factors to promoters. Component of nf-munr repressor; binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. Represses T-cell receptor (TCR) β enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR β enhancer. Binds to the TH enhancer; may require the basic helix-loop-helix protein TCF4 as a coactivator (By similarity).

Synonyms:

Homeobox protein cut-like 1 (CCAAT displacement protein) (CDP) (Homeobox protein cux-1), CUX1, CUTL1

UniProt Protein Function:	CUTL1: Probably has a broad role in mammalian development as a repressor of developmentally regulated gene expression. May act by preventing binding of positively-activing CCAAT factors to promoters. Component of nf-munr repressor; binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. Represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Belongs to the CUT homeobox family. 8 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:DNA-binding; Membrane protein, integral; Transcription factor Chromosomal Location of Human Ortholog: 7q22.1 Cellular Component: integral to Golgi membrane; nucleus; cytosol Molecular Function:sequence-specific DNA binding; chromatin binding Biological Process: regulation of transcription from RNA polymerase II promoter; intra-Golgi vesicle-mediated transport; transcription, DNA-dependent; multicellular organismal development; auditory receptor cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of dendrite morphogenesis; negative regulation of transcription from RNA polymerase II promoter; kidney development; lung development
NCBI Summary:	The protein encoded by this gene is a member of the homeodomain family of DNA binding proteins. It may regulate gene expression, morphogenesis, and differentiation and it may also play a role in the cell cycle progession. Several alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Feb 2011]
UniProt Code:	P39880
NCBI GenInfo Identifier:	296439379
NCBI Gene ID:	1523
NCBI Accession:	P39880.3
UniProt Secondary Accession:	P39880,Q6NYH4, Q75LE5, Q75MT2, Q75MT3, Q86UJ7, Q9UEV5 B3KV79, J3KQV9,
UniProt Related Accession:	P39880,Q13948
Molecular Weight:	77 kDa
NCBI Full Name:	Homeobox protein cut-like 1
NCBI Synonym Full Names:	cut-like homeobox 1
NCBI Official Symbol:	CUX1
NCBI Official Synonym Symbols:	CDP; CUX; p75; CASP; CDP1; COY1; Clox; p100; p110; p200; CUTL1; GOLIM6; CDP/Cut; Cux/CDP; Nbla10317
NCBI Protein Information:	protein CASP; cut homolog; homeobox protein cux-1; CCAAT displacement protein; golgi integral membrane protein 6; putative protein product of Nbla10317
UniProt Protein Name:	Homeobox protein cut-like 1
UniProt Synonym Protein Names:	CCAAT displacement protein; CDP; Homeobox protein cux-1
Protein Family:	Homeobox protein
UniProt Gene Name:	CUX1
UniProt Entry Name:	CUX1_HUMAN

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)