The Cullin-3 Polyclonal Antibody (CAB1078) is a valuable tool for researchers studying the function of Cullin-3, a critical component of the Cullin-RING E3 ubiquitin ligase complex responsible for protein degradation. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to Cullin-3 protein, this antibody enables precise detection and analysis in various cell types, making it an essential reagent for studies in cell biology and protein degradation mechanisms.Cullin-3 is known to play a pivotal role in regulating cellular processes such as cell cycle progression, signal transduction, and protein quality control through targeted protein degradation.
Dysregulation of Cullin-3 function has been implicated in various diseases, including cancer and neurodegenerative disorders. Research into the molecular mechanisms involving Cullin-3 is crucial for understanding its role in disease pathogenesis and identifying potential therapeutic targets for intervention. The Cullin-3 Polyclonal Antibody provides researchers with a powerful tool to unravel the complexities of Cullin-3 function and its impact on cellular physiology.
Product Name:
Cullin 3 Rabbit Polyclonal Antibody
SKU:
CAB1078
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 468-768 of human Cullin 3 (NP_003581.1).
This gene encodes a member of the cullin protein family. The encoded protein plays a critical role in the polyubiquitination and subsequent degradation of specific protein substrates as the core component and scaffold protein of an E3 ubiquitin ligase complex. Complexes including the encoded protein may also play a role in late endosome maturation. Mutations in this gene are a cause of type 2E pseudohypoaldosteronism. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method:
Affinity purification
Gene ID:
8452
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Cullin 3 antibody (CAB1078) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
