The Cullin-2 Polyclonal Antibody (CAB5308) offered by AssayGenie is specifically designed for research involving Cullin-2, a key protein involved in the ubiquitin-proteasome pathway. This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. By targeting the Cullin-2 protein, this antibody enables researchers to detect and analyze its expression in various cell types, making it an essential tool for studies in cell biology, cancer research, and protein degradation pathways.Cullin-2 is a vital component of the Cullin-RING E3 ubiquitin ligase complex, which plays a crucial role in protein degradation and regulation of cellular processes.
Dysregulation of the ubiquitin-proteasome pathway, including alterations in Cullin-2 activity, has been implicated in various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases. Therefore, studying the function and expression of Cullin-2 is essential for understanding its role in disease development and progression.Overall, the Cullin-2 Polyclonal Antibody offered by AssayGenie provides researchers with a reliable tool for investigating the functions of Cullin-2 and its implications in cellular processes and disease pathology. Its high specificity and sensitivity make it a valuable asset for advancing research in the field of molecular biology and therapeutic development.
Product Name:
Cullin 2 Rabbit Polyclonal Antibody
SKU:
CAB5308
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 40-310 of human Cullin 2 (NP_001185708.1).
Enables ubiquitin protein ligase binding activity. Predicted to be involved in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process and protein ubiquitination. Predicted to act upstream of or within protein catabolic process. Located in nucleoplasm. Part of Cul2-RING ubiquitin ligase complex.
Purification Method:
Affinity purification
Gene ID:
8453
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Cullin 2 antibody (CAB5308) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
