The Cullin-1 Rabbit Monoclonal Antibody (CAB19034) is specifically designed for research involving Cullin-1, a key component of the SCF (Skp1-Cullin 1-F-box) E3 ubiquitin ligase complex that plays a crucial role in protein degradation. This antibody, produced in rabbits, is highly reactive with human samples and has been validated for use in various applications such as Western blotting.Cullin-1 is involved in the regulation of cell cycle progression, signal transduction, and tumorigenesis, making it a target of interest in cancer research. By detecting and analyzing the Cullin-1 protein in different cell types, researchers can gain insight into its function and potential therapeutic implications in cancer treatment.
Furthermore, the Cullin-1 Rabbit Monoclonal Antibody is an essential tool for studying the molecular mechanisms underlying protein degradation and identifying potential drug targets for various diseases. Its high specificity and sensitivity make it a valuable asset for immunology and cancer research, providing researchers with a reliable means of investigating the role of Cullin-1 in cellular processes.
Product Name:
Cullin 1 Rabbit Monoclonal Antibody
SKU:
CAB19034
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 677-776 of human Cullin 1 (Q13616).
Predicted to enable ubiquitin protein ligase binding activity and ubiquitin-protein transferase activity. Involved in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process and protein ubiquitination. Located in plasma membrane. Part of Parkin-FBXW7-Cul1 ubiquitin ligase complex and SCF ubiquitin ligase complex.
Purification Method:
Affinity purification
Gene ID:
8454
Clone Number:
ARC0436
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Cullin 1 antibody (CAB19034) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
