The Cullin-1 Polyclonal Antibody is designed for researchers studying Cullin-1, a key component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex involved in protein degradation. This antibody, produced in rabbits, is highly specific for detecting Cullin-1 in human samples and has been validated for use in Western blot and immunohistochemistry applications. By binding to the Cullin-1 protein, this antibody allows for the precise detection and analysis of Cullin-1 expression in various cell types.Cullin-1 plays a critical role in regulating the degradation of target proteins, thereby controlling important cellular processes such as cell cycle progression, signaling pathways, and DNA replication.
Dysfunction of Cullin-1 has been implicated in various diseases, including cancer and neurodegenerative disorders, making it a promising target for therapeutic intervention. By using the Cullin-1 Polyclonal Antibody, researchers can further investigate the role of Cullin-1 in disease development and progression, advancing our understanding of this critical cellular pathway.
Product Name:
Cullin 1 Polyclonal Antibody
SKU:
CAB21687
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 435-593 of human Cullin 1 (NP_003583.2).
Predicted to enable ubiquitin protein ligase binding activity and ubiquitin-protein transferase activity. Involved in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process and protein ubiquitination. Located in plasma membrane. Part of Parkin-FBXW7-Cul1 ubiquitin ligase complex and SCF ubiquitin ligase complex.
Purification Method:
Affinity purification
Gene ID:
8454
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Cullin 1 Rabbit pAb (CAB21687) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
