The Cullin-5 Polyclonal Antibody (CAB5369) is a vital tool for researchers studying the Cullin-5 protein, a key component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.Cullin-5 is involved in the regulation of protein degradation through the ubiquitin-proteasome system, governing the stability of numerous target proteins that play roles in various cellular processes. The ability to specifically target and detect Cullin-5 with this antibody allows for precise analysis and characterization in different cell types, making it indispensable for investigations in cell biology, molecular biology, and cancer research.
The precise regulation of protein turnover by Cullin-5 underscores its importance in cellular homeostasis and the maintenance of normal physiological functions. Dysregulation of Cullin-5 activity has been implicated in the pathogenesis of certain diseases, particularly cancer, making it a promising target for therapeutic interventions. Therefore, the Cullin-5 Polyclonal Antibody serves as a valuable tool for dissecting the functions and mechanisms of Cullin-5, paving the way for potential advancements in the development of targeted therapies for various disorders.
Product Name:
CUL5 Rabbit Polyclonal Antibody
SKU:
CAB5369
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CUL5 (NP_003469.2).
Enables ubiquitin protein ligase binding activity. Predicted to be involved in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process and protein ubiquitination. Predicted to act upstream of or within cerebral cortex radially oriented cell migration and radial glia guided migration of Purkinje cell. Located in site of DNA damage. Part of Cul5-RING ubiquitin ligase complex.
Purification Method:
Affinity purification
Gene ID:
8065
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat heart, using CUL5 Rabbit pAb (CAB5369) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
