The CUL3 Polyclonal Antibody (CAB16455) is a valuable tool for researchers studying CUL3, a key component of the Cullin-RING E3 ubiquitin ligase complex involved in the regulation of protein degradation. This antibody, generated in rabbits, displays high reactivity with human samples and has been validated for use in Western blot applications.CUL3 plays a crucial role in the ubiquitin-proteasome system, targeting specific proteins for degradation and thereby influencing various cellular processes such as cell cycle progression, signal transduction, and DNA repair. The CUL3 Polyclonal Antibody binds to the CUL3 protein, enabling accurate detection and analysis in a variety of cell types.
Research involving CUL3 is essential for understanding its role in cellular homeostasis, protein turnover, and disease development. Dysregulation of the CUL3 pathway has been implicated in cancer, neurodegenerative diseases, and metabolic disorders, making this antibody a valuable tool for investigating potential therapeutic targets and pathways. By studying CUL3, researchers can gain insights into the molecular mechanisms underlying these conditions and potentially develop new treatment strategies.
Product Name:
Cullin 3 Rabbit Polyclonal Antibody
SKU:
CAB16455
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 569-768 of human Cullin 3 (NP_003581.1).
This gene encodes a member of the cullin protein family. The encoded protein plays a critical role in the polyubiquitination and subsequent degradation of specific protein substrates as the core component and scaffold protein of an E3 ubiquitin ligase complex. Complexes including the encoded protein may also play a role in late endosome maturation. Mutations in this gene are a cause of type 2E pseudohypoaldosteronism. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method:
Affinity purification
Gene ID:
8452
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Cullin 3 Rabbit pAb (CAB16455) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
