The CTNNB1 Polyclonal Antibody (CAB11932) is a valuable tool for researchers studying the CTNNB1 protein, also known as beta-catenin, which plays a crucial role in the Wnt signaling pathway and cell adhesion. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot and immunohistochemistry applications.CTNNB1 is a key player in cell proliferation, differentiation, and migration, making it a target of interest in cancer research and developmental biology. Aberrant activation of CTNNB1 has been implicated in the progression of various cancers, including colorectal cancer and hepatocellular carcinoma.
By using the CTNNB1 Polyclonal Antibody, researchers can detect and analyze the expression of CTNNB1 in different cell types and tissue samples, providing important insights into its role in various biological processes. This antibody is a valuable tool for studying the molecular mechanisms underlying cancer development and progression, as well as potential therapeutic targets for cancer treatment.
Product Name:
β-Catenin Rabbit Polyclonal Antibody
SKU:
CAB11932
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human beta Catenin (NP_001895.1).
Cell junction, Cell membrane, Cytoplasm, Nucleus, adherens junction, centrosome, cytoskeleton, microtubule organizing center, spindle pole.
Calculated MW:
85kDa
Observed MW:
100kDa
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
1499
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using β-Catenin antibody (CAB11932) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
