The beta-Catenin Antibody (CAB11932) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot and immunohistochemistry applications.CTNNB1 is a key player in cell proliferation, differentiation, and migration, making it a target of interest in cancer research and developmental biology. Aberrant activation of CTNNB1 has been implicated in the progression of various cancers, including colorectal cancer and hepatocellular carcinoma.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
beta-Catenin Antibody
SKU:
CAB11932
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
EVR7, CTNNB, MRD19, NEDSDV, armadillo, β-Catenin
Positive Sample:
Mouse brain, Rat brain
Cellular Localization:
Cell Junction, Cell Membrane, Cytoplasm, Nucleus, Adherens Junction, Centrosome, Cytoskeleton, Microtubule Organizing Center, Spindle Pole.
Calculated MW:
85kDa
Observed MW:
100kDa
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
1499
RRID
AB_2758875
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using β-Catenin Rabbit pAb (CAB11932) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using β-Catenin Rabbit pAb (CAB11932) at dilution of 1:100. Blue: DAPI for nuclear staining.
