The CTLA4 Polyclonal Antibody (CAB2063) is specifically designed for research involving CTLA4, a crucial immune checkpoint molecule that plays a key role in regulating T cell activation and maintaining immune tolerance. This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in various applications such as Western blot and immunohistochemistry.CTLA4, also known as cytotoxic T-lymphocyte-associated protein 4, is a checkpoint protein that negatively regulates T cell activation and prevents autoimmunity by inhibiting excessive immune responses. Dysregulation of CTLA4 has been implicated in various diseases, including cancer and autoimmune disorders, making it an important target for research in immunology and oncology.
By targeting the CTLA4 protein, researchers can gain valuable insights into the mechanisms of immune regulation and potentially develop novel therapies for modulating immune responses in diseases such as cancer and autoimmunity. The CTLA4 Polyclonal Antibody (CAB2063) is a valuable tool for studying CTLA4 expression and function in various cell types, providing important information for advancing our understanding of immune modulation and developing targeted immunotherapies.
Product Name:
CTLA4 Rabbit Polyclonal Antibody
SKU:
CAB2063
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 36-161 of human CD152/CTLA-4 (NP_005205.2).
Cell membrane, Single-pass type I membrane protein.
Calculated MW:
25kDa
Observed MW:
30kDa
This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.
Purification Method:
Affinity purification
Gene ID:
1493
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using CD152/CTLA-4 Rabbit pAb (CAB2063) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
