The CTLA4 Polyclonal Antibody (CAB13966) is a valuable tool for researchers studying CTLA4, a critical immune checkpoint protein involved in regulating T cell activation and immune response. Raised in rabbits, this antibody is highly specific to human samples and has been validated for use in Western blot applications.CTLA4, also known as cytotoxic T-lymphocyte-associated protein 4, is a key player in immune tolerance and prevention of autoimmunity by downregulating T cell activation. Dysregulation of CTLA4 is associated with various autoimmune diseases and cancer, making it a promising target for therapeutic interventions.
This polyclonal antibody binds specifically to CTLA4, allowing for accurate detection and analysis of its expression levels in different cell types. Its versatility and reliability make it an essential tool for research in immunology, oncology, and autoimmune disorders. By understanding the role of CTLA4 in immune regulation, researchers can develop novel strategies for treating diseases characterized by immune dysfunction.
Product Name:
CTLA4 Rabbit Polyclonal Antibody
SKU:
CAB13966
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CD152/CTLA-4 (NP_005205.2).
Cell membrane, Single-pass type I membrane protein.
Calculated MW:
25kDa
Observed MW:
50kDa
This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.
Purification Method:
Affinity purification
Gene ID:
1493
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of THP1 cells, using CD152/CTLA-4 antibody (CAB13966) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
