The CTLA4 Polyclonal Antibody (CAB13965) is a valuable tool for researchers studying CTLA4, a key immune checkpoint molecule that plays a crucial role in regulating T cell activation and immune responses. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in a variety of applications, including Western blotting.CTLA4, also known as cytotoxic T lymphocyte-associated protein 4, is an essential negative regulator of T cell activation and is involved in maintaining immune tolerance and preventing autoimmunity. Its dysregulation has been linked to various diseases, including cancer and autoimmune disorders, making it an attractive target for therapeutic intervention.
By utilizing the CTLA4 Polyclonal Antibody, researchers can effectively detect and analyze CTLA4 protein expression in different cell types, providing valuable insights into the role of CTLA4 in immune modulation and disease pathogenesis. This antibody is an indispensable tool for studies in immunology, oncology, and autoimmune research, ultimately contributing to the development of novel therapeutic strategies targeting CTLA4 signaling pathways.
Product Name:
CTLA4 Rabbit Polyclonal Antibody
SKU:
CAB13965
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 49-223 of human CD152/CTLA-4 (NP_005205.2).
Cell membrane, Single-pass type I membrane protein.
Calculated MW:
25kDa
Observed MW:
36kDa
This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.
Purification Method:
Affinity purification
Gene ID:
1493
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CD152/CTLA-4 antibody (CAB13965) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
