The CTCFL Polyclonal Antibody (CAB6149) is a valuable tool for researchers studying the CTCFL protein, a key player in gene regulation, especially in cancer and embryonic development. This antibody, produced in rabbits, has been rigorously tested for reactivity with human samples and is suitable for use in a variety of applications, including Western blotting. CTCFL, also known as CCCTC-binding factor-like protein, is involved in regulating gene expression by binding to specific DNA sequences.
Its role in cancer and embryogenesis makes it a promising target for further research into disease mechanisms and potential therapeutic interventions. By using the CTCFL Polyclonal Antibody, researchers can accurately detect and analyze CTCFL expression in various cell types, advancing our understanding of its function in health and disease.
Product Name:
CTCFL Rabbit Polyclonal Antibody
SKU:
CAB6149
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human CTCFL (NP_001255970.1).
CCCTC-binding factor (CTCF), an 11-zinc-finger factor involved in gene regulation, utilizes different zinc fingers to bind varying DNA target sites. CTCF forms methylation-sensitive insulators that regulate X-chromosome inactivation. This gene is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes, unlike CTCF which is expressed primarily in the nucleus of somatic cells. CTCF and the protein encoded by this gene are normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
140690
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CTCFL antibody (CAB6149) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 8s.
