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CTCFL Rabbit Polyclonal Antibody (CAB6149)

SKU:
CAB6149
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle
  • Cell Cycle Antibodies 1 Anti-CTCFL Antibody CAB6149
  • Cell Cycle Antibodies 1 Anti-CTCFL Antibody CAB6149
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:CTCFL Rabbit Polyclonal Antibody
SKU:CAB6149
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human CTCFL (NP_001255970.1).
Sequence:MAAT EISV LSEQ FTKI KELE LMPE KGLK EEEK DGVC REKD HRSP SELE AERT SGAF QDSV LEEE VELV LAPS EESE KYIL TLQT VHFT SEAV ELQD MSLL SIQQ QEGV QVVV QQPG PGLL WLEE GPRQ SLQQ CVAI SIQQ ELYS PQEM EVLQ FHAL EENV MVAS EDSK LAVS LAET TGLI KLEE EQEK NQLL AERT KEQL FFVE TMSG DERS DEIV LTVS NSNV EEQE DQPT AGQA DAEK AKST KNQR KTKG AKGT FHCD VCMF TSSR MS
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:CT27; BORIS; CTCF-T; HMGB1L1; dJ579F20.2; CTCFL
Positive Sample:Mouse testis
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:76kDa
Observed MW:83kDa

CCCTC-binding factor (CTCF), an 11-zinc-finger factor involved in gene regulation, utilizes different zinc fingers to bind varying DNA target sites. CTCF forms methylation-sensitive insulators that regulate X-chromosome inactivation. This gene is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes, unlike CTCF which is expressed primarily in the nucleus of somatic cells. CTCF and the protein encoded by this gene are normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Purification Method:Affinity purification
Gene ID:140690
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using CTCFL antibody (CAB6149) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 8s.

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