The CTCF Polyclonal Antibody (CAB13272) is a specialized research tool designed for investigating the role of CTCF, a key transcriptional regulator involved in gene expression and chromatin organization. This antibody, produced in rabbits, exhibits strong reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the CTCF protein, this antibody facilitates the detection and analysis of CTCF in various cell types, making it an essential component for studies in genetics, epigenetics, and cancer research.CTCF is a critical protein that plays a pivotal role in the regulation of gene expression by facilitating interactions between DNA sequences and influencing chromatin structure. Its involvement in various cellular processes, including DNA methylation, chromatin looping, and genomic stability, underscores its significance as a target for investigation in diverse fields of study.
Researchers interested in understanding the mechanisms underlying gene regulation, DNA organization, and disease development will benefit from the use of this antibody in their experiments.Overall, the CTCF Polyclonal Antibody (CAB13272) offers a valuable tool for exploring the functions and molecular interactions of CTCF, providing researchers with the means to uncover new insights into gene regulation and chromatin dynamics. Its versatility and reliability make it an indispensable resource for advancing scientific knowledge in the fields of molecular biology, genetics, and oncology.
Product Name:
CTCF Rabbit Polyclonal Antibody
SKU:
CAB13272
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CTCF (NP_006556.1).
WB,1:1000 - 1:2000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cellsChIP,3μg antibody for 10μg-15μg of Chromatin
Synonyms:
MRD21; FAP108; CFAP108; CTCF
Positive Sample:
293T
Conjugate:
Unconjugated
Cellular Localization:
Chromosome, Nucleus, centromere, nucleoplasm.
Calculated MW:
83kDa
Observed MW:
140kDa
This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
10664
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using CTCF Rabbit pAb (CAB13272) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
