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CTCF Rabbit Polyclonal Antibody (CAB13272)

SKU:
CAB13272
Product Type:
Antibody
Applications:
WB
IHC
IF
IP
ChIP
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
  • Epigenetics and Nuclear Signaling Antibodies 1 Anti-CTCF Antibody CAB13272
  • Epigenetics and Nuclear Signaling Antibodies 1 Anti-CTCF Antibody CAB13272
  • Epigenetics and Nuclear Signaling Antibodies 1 Anti-CTCF Antibody CAB13272
  • Epigenetics and Nuclear Signaling Antibodies 1 Anti-CTCF Antibody CAB13272
  • Epigenetics and Nuclear Signaling Antibodies 1 Anti-CTCF Antibody CAB13272
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:CTCF Rabbit Polyclonal Antibody
SKU:CAB13272
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CTCF (NP_006556.1).
Sequence:MEGD AVEA IVEE SETF IKGK ERKT YQRR REGG QEED ACHL PQNQ TDGG EVVQ DVNS SVQM VMME QLDP TLLQ MKTE VMEG TVAP EAEA AVDD TQII TLQV VNME EQPI NIGE LQLV QVPV PVTV PVAT TSVE ELQG AYEN EVSK EGLA ESEP MICH TLPL PEGF QVVK VGAN GEVE TLEQ GELP PQED PSWQ KDPD YQPP AKKT KKTK KSKL RYTE EGKD VDVS VYDF EEEQ QEGL LSEV NAEK VVGN MKPP KPTK IKKK
Tested Applications:WB IHC-P IF/ICC IP ChIP ELISA
Recommended Dilution:WB,1:1000 - 1:2000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cellsChIP,3μg antibody for 10μg-15μg of Chromatin
Synonyms:MRD21; FAP108; CFAP108; CTCF
Positive Sample:293T
Conjugate:Unconjugated
Cellular Localization:Chromosome, Nucleus, centromere, nucleoplasm.
Calculated MW:83kDa
Observed MW:140kDa

This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Purification Method:Affinity purification
Gene ID:10664
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from 293T cells, using CTCF Rabbit pAb (CAB13272) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

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