The CTCF Polyclonal Antibody (CAB1133) is specially designed for research involving the CTCF protein, a key regulatory factor in gene expression and genome organization. This antibody, raised in rabbits, has high reactivity with human samples and is validated for use in various applications, including Western blotting.CTCF, also known as CCCTC-binding factor, plays a crucial role in chromatin organization and gene regulation by binding to specific DNA sequences. Its ability to insulate and regulate gene expression makes it a valuable target for studies in epigenetics, gene transcription, and genome function.
Understanding the function and activity of CTCF is essential for unraveling the complexities of gene regulation and its impact on cellular functions.The CTCF Polyclonal Antibody enables researchers to detect and analyze CTCF protein levels in different cell types, providing valuable insights into its role in various biological processes. Whether studying gene expression dynamics, chromatin architecture, or regulatory pathways, this antibody is a powerful tool for advancing research in genetics, epigenetics, and molecular biology.
Product Name:
CTCF Rabbit Polyclonal Antibody
SKU:
CAB1133
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CTCF (NP_006556.1).
WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cellsChIP,5μg antibody for 10μg-15μg of Chromatin
Synonyms:
MRD21; FAP108; CFAP108; CTCF
Positive Sample:
HeLa,Mouse thymus
Conjugate:
Unconjugated
Cellular Localization:
Chromosome, Nucleus, centromere, nucleoplasm.
Calculated MW:
83kDa
Observed MW:
140kDa
This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
10664
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using CTCF Rabbit pAb (CAB1133) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
