CTCF Rabbit Polyclonal Antibody (CAB1133)
- SKU:
- CAB1133
- Product Type:
- Antibody
- Applications:
- WB
- IHC
- IF
- IP
- ChIP
- Reactivity:
- Human
- Mouse
- Rat
- Monkey
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
Product Name: | CTCF Rabbit Polyclonal Antibody |
SKU: | CAB1133 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CTCF (NP_006556.1). |
Sequence: | MEGD AVEA IVEE SETF IKGK ERKT YQRR REGG QEED ACHL PQNQ TDGG EVVQ DVNS SVQM VMME QLDP TLLQ MKTE VMEG TVAP EAEA AVDD TQII TLQV VNME EQPI NIGE LQLV QVPV PVTV PVAT TSVE ELQG AYEN EVSK EGLA ESEP MICH TLPL PEGF QVVK VGAN GEVE TLEQ GELP PQED PSWQ KDPD YQPP AKKT KKTK KSKL RYTE EGKD VDVS VYDF EEEQ QEGL LSEV NAEK VVGN MKPP KPTK IKKK |
Tested Applications: | WB IF/ICC IP ChIP ELISA |
Recommended Dilution: | WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cellsChIP,5μg antibody for 10μg-15μg of Chromatin |
Synonyms: | MRD21; FAP108; CFAP108; CTCF |
Positive Sample: | HeLa,Mouse thymus |
Conjugate: | Unconjugated |
Cellular Localization: | Chromosome, Nucleus, centromere, nucleoplasm. |
Calculated MW: | 83kDa |
Observed MW: | 140kDa |
This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method: | Affinity purification |
Gene ID: | 10664 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |