The CSTB Polyclonal Antibody (CAB19961) is a versatile tool for research involving CSTB, a protein known for its role in various cellular processes such as apoptosis and autophagy. This antibody, produced in rabbits, is highly specific and reactive with human samples, making it suitable for use in Western blot applications. CSTB is a member of the cystatin superfamily and is involved in the regulation of protease activity within cells. Dysregulation of CSTB has been implicated in diseases such as cancer, neurodegenerative disorders, and inflammatory conditions.
By targeting CSTB with this antibody, researchers can study its expression and function in different cell types, providing valuable insights into its role in disease pathology.This antibody is a valuable tool for scientists conducting research in areas such as cancer biology, neurobiology, and immunology, to further understand the mechanisms underlying CSTB function and its potential as a therapeutic target. Its specificity and reliability make it an essential component for studies aiming to unravel the complexities of CSTB-mediated processes.
Product Name:
[KO Validated] CSTB Rabbit Polyclonal Antibody
SKU:
CAB19961
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-98 of human CSTB (NP_000091.1).
The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). One type of mutation responsible for EPM1 is the expansion in the promoter region of this gene of a CCCCGCCCCGCG repeat from 2-3 copies to 30-78 copies.
Purification Method:
Affinity purification
Gene ID:
1476
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and CSTB knockout (KO) 293T cells, using [KO Validated] CSTB Rabbit pAb (CAB19961) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
