The CST5 Polyclonal Antibody (PAC063283) is a valuable tool for researchers studying CST5, a member of the cystatin superfamily implicated in various biological processes, including regulation of protease activity and immune response modulation. Raised in rabbits, this antibody is highly specific for human samples and has been validated for use in Western blot applications. By binding to the CST5 protein, researchers can detect and analyze its expression levels in different cell types, making it a versatile tool for studies in immunology, cancer research, and beyond.
CST5, also known as cystatin D, is involved in the regulation of protein degradation and has been linked to diseases such as cancer, neurodegenerative disorders, and inflammatory conditions. Understanding the role of CST5 in these contexts is essential for developing targeted therapies and diagnostic tools. By using the CST5 Polyclonal Antibody, researchers can gain valuable insights into the function of CST5 and its potential as a therapeutic target.
IHC image of PACO63283 diluted at 1:500 and staining in paraffin-embedded human salivary gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Cysteine proteinase inhibitor that possibly plays a protective role against proteinases present in the oral cavity. The order of preference for inhibition is cathepsin S > cathepsin H > cathepsin L > cathepsin B.
Synonyms:
Cystatin-D (Cystatin-5), CST5
UniProt Protein Function:
CST5: Cysteine proteinase inhibitor that possibly plays a protective role against proteinases present in the oral cavity. The order of preference for inhibition is cathepsin S > cathepsin H > cathepsin L > cathepsin B. Belongs to the cystatin family.Protein type: Secreted, signal peptide; SecretedChromosomal Location of Human Ortholog: 20p11.21Cellular Component: extracellular regionMolecular Function: protein binding; cysteine protease inhibitor activity
UniProt Protein Details:
NCBI Summary:
The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a protein found in saliva and tears. The encoded protein may play a protective role against proteinases present in the oral cavity. [provided by RefSeq, Jul 2008]
