The CST3 Polyclonal Antibody (CAB13291) is an essential tool for researchers studying CST3, a cysteine proteinase inhibitor with various biological functions. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. It specifically binds to the CST3 protein, allowing for precise detection and analysis in a variety of cell types. This antibody is particularly well-suited for investigations in fields such as protease biology, inflammation, and neurodegenerative diseases.
CST3, also known as cystatin C, is an important regulator of protease activity in the body, helping to maintain protein homeostasis and prevent tissue damage. Dysregulation of CST3 is associated with a range of pathological conditions, including Alzheimer's disease and cardiovascular disorders. By studying the role of CST3 using this antibody, researchers can gain insights into the molecular mechanisms underlying these diseases and potentially identify novel therapeutic targets.
Product Name:
Cystatin C Rabbit Polyclonal Antibody
SKU:
CAB13291
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 27-146 of human Cystatin C (NP_000090.1).
The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes the most abundant extracellular inhibitor of cysteine proteases, which is found in high concentrations in biological fluids and is expressed in virtually all organs of the body. A mutation in this gene has been associated with amyloid angiopathy. Expression of this protein in vascular wall smooth muscle cells is severely reduced in both atherosclerotic and aneurysmal aortic lesions, establishing its role in vascular disease. In addition, this protein has been shown to have an antimicrobial function, inhibiting the replication of herpes simplex virus. Alternative splicing results in multiple transcript variants encoding a single protein.
Purification Method:
Affinity purification
Gene ID:
1471
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Cystatin C antibody (CAB13291) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 5s.
