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NG2/CSPG4 Monoclonal Antibody (CAB21095)

SKU:
CAB21095
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Universal
Host Species:
Rabbit
Isotype:
IgG
$215 - $563
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:NG2/CSPG4 Monoclonal Antibody
SKU:CAB21095
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1340-1563 of human NG2/CSPG4 (NP_001888.2).
Sequence:LGAP LEGV LVEL EVLP AAIP LEAQ NFSV PEGG SLTL APPL LRVS GPYF PTLL GLSL QVLE PPQH GALQ KEDG PQAR TLSA FSWR MVEE QLIR YVHD GSET LTDS FVLM ANAS EMDR QSHP VAFT VTVL PVND QPPI LTTN TGLQ MWEG ATAP IPAE ALRS TDGD SGSE DLVY TIEQ PSNG RVVL RGAP GTEV RSFT QAQL DGGL VLFS HRGT LDGG FRFR LSDG
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:NG2; MCSP; MCSPG; MSK16; CSPG4A; HMW-MAA; MEL-CSPG; NG2/CSPG4
Positive Sample:A375
Conjugate:Unconjugated
Cellular Localization:Apical cell membrane, Cell projection, Extracellular side, Single-pass type I membrane protein, lamellipodium membrane.
Calculated MW:251kDa
Observed MW:250-450kDa

A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.

Purification Method:Affinity purification
Gene ID:1464
Clone Number:ARC52162
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of A375, using NG2/CSPG4 antibody (CAB21095) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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