The CSF2 Polyclonal Antibody (CAB6127) is a vital tool for researchers studying CSF2, a cytokine known for its role in immune regulation and inflammation modulation. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot assays. By binding specifically to the CSF2 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an ideal choice for studies in immunology and cancer research.CSF2, also known as Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF), is essential for the development and function of various immune cells, including granulocytes, macrophages, and dendritic cells.
Its involvement in regulating immune responses and inflammatory processes makes it a valuable target for investigating diseases such as cancer, autoimmune disorders, and inflammatory conditions. By understanding the mechanisms of action of CSF2, researchers can potentially uncover new therapeutic strategies for manipulating immune responses in these disease contexts.
Product Name:
CSF2 Rabbit Polyclonal Antibody
SKU:
CAB6127
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CSF2 (NP_000749.2).
Sequence:
ARSP SPST QPWE HVNA I
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200
Synonyms:
CSF; GMCSF; CSF2
Positive Sample:
Jurkat,A-549,Mouse thymus
Conjugate:
Unconjugated
Cellular Localization:
Secreted.
Calculated MW:
16kDa
Observed MW:
16kDa
The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. This gene plays a role in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been detected in SARS-CoV-2 infected patients that develop acute respiratory distress syndrome. Mice deficient in this gene or its receptor develop pulmonary alveolar proteinosis.
Purification Method:
Affinity purification
Gene ID:
1437
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CSF2 antibody (CAB6127) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 120s.
