The CSDE1 Polyclonal Antibody (CAB5941) is a vital tool for researchers studying CSDE1, a RNA-binding protein involved in various cellular processes, including RNA metabolism and translation regulation. This antibody, produced in rabbits, exhibits high affinity and specificity for CSDE1 in human samples, making it suitable for use in techniques such as Western blotting. By binding to the CSDE1 protein, this antibody facilitates the detection and analysis of CSDE1 in diverse cell types, making it ideal for investigations in molecular biology and cancer research.
CSDE1, also known as Cold Shock Domain-Containing E1, is a key player in post-transcriptional gene regulation, influencing mRNA stability and translation efficiency. Its role in cellular function and disease makes it an attractive target for studies on cancer, neurodegenerative disorders, and viral infections. By elucidating the functions of CSDE1, researchers can gain insights into disease mechanisms and potentially identify novel therapeutic strategies targeting this protein.
Product Name:
[KO Validated] CSDE1 Rabbit Polyclonal Antibody
SKU:
CAB5941
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 369-600 of human CSDE1 (NP_009089.4).
WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
UNR; D1S155E; E1
Positive Sample:
HeLa
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm.
Calculated MW:
89kDa
Observed MW:
89kDa
Enables RNA stem-loop binding activity. Involved in IRES-dependent viral translational initiation; nuclear-transcribed mRNA catabolic process, no-go decay; and stress granule assembly. Located in Golgi apparatus; cytosol; and plasma membrane. Part of CRD-mediated mRNA stability complex.
Purification Method:
Affinity purification
Gene ID:
7812
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and CSDE1 knockout (KO) HeLa cells, using [KO Validated] CSDE1 Rabbit pAb (CAB5941) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.
