The CRYYA Polyclonal Antibody (CAB5725) is a high-quality research tool designed for the detection and analysis of CRYYA, a protein known to be involved in eye development and cataract formation. This antibody, raised in rabbits, is specifically reactive with human samples and has been validated for use in Western blot applications. By binding to the CRYYA protein, researchers can study its expression and function in various cell types, making it a valuable tool for studies in ophthalmology and developmental biology.CRYYA, also referred to as Alpha-Crystallin A Chain, plays a crucial role in maintaining the transparency and refractive index of the lens in the eye.
Changes in the expression or function of CRYYA have been associated with cataract formation, a common age-related eye disorder. By investigating the role of CRYYA in eye development and disease, researchers can gain insights into potential therapeutic targets for treating cataracts and other eye-related conditions.Overall, the CRYYA Polyclonal Antibody (CAB5725) is an essential tool for researchers interested in studying the function of CRYYA in eye development and cataract formation. Its high specificity and sensitivity make it a reliable choice for Western blot analysis and other experimental techniques in the field of ophthalmology.
Product Name:
CRYAA Rabbit Polyclonal Antibody
SKU:
CAB5725
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Zebrafish
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-173 of human CRYAA (NP_000385.1).
Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC).
Purification Method:
Affinity purification
Gene ID:
1409
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HepG2 cells, using CRYAA Rabbit pAb (CAB5725) at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
