The CRTC2 Polyclonal Antibody is a valuable tool for researchers studying the CRTC2 protein, which is involved in transcriptional regulation and cell metabolism. This rabbit polyclonal antibody is highly specific to human samples and has been validated for use in various applications, including Western blotting. By binding to the CRTC2 protein, this antibody enables the detection and analysis of CRTC2 in a variety of cell types, making it ideal for studies in cellular biology and metabolism research.
CRTC2, also known as CREB-regulated transcription coactivator 2, plays a crucial role in regulating gene expression in response to intracellular signals such as calcium and cAMP. Its involvement in cellular metabolism and energy homeostasis makes it a key target for research into conditions like diabetes, obesity, and cancer. Understanding the functions of CRTC2 can provide valuable insights into metabolic regulation and potential therapeutic targets for metabolic disorders.
Product Name:
CRTC2 Rabbit Polyclonal Antibody
SKU:
CAB10682
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 16-126 of human CRTC2 (NP_859066.1).
This gene encodes a member of the transducers of regulated cAMP response element-binding protein activity family of transcription coactivators. These proteins promote the transcription of genes targeted by the cAMP response element-binding protein, and therefore play an important role in many cellular processes. Under basal conditions the encoded protein is phosphorylated by AMP-activated protein kinase or the salt-inducible kinases and is sequestered in the cytoplasm. Upon activation by elevated cAMP or calcium, the encoded protein translocates to the nucleus and increases target gene expression. Single nucleotide polymorphisms in this gene may increase the risk of type 2 diabetes. A pseudogene of this gene is located on the long arm of chromosome 5.
Purification Method:
Affinity purification
Gene ID:
200186
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CRTC2 antibody (CAB10682) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
