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Crotonyl-HIST1H2BC (K20) Antibody (PACO60477)

SKU:
PACO60477
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
ELISA
WB
IF
ChIP
Antibody Type:
Polyclonal Antibody
Conjugation:
Unconjugated
  • Crotonyl-HIST1H2BC K20 Antibody PACO60477
  • Crotonyl-HIST1H2BC K20 Antibody PACO60477
  • Crotonyl-HIST1H2BC K20 Antibody PACO60477
€349
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Description

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Crotonyl-HIST1H2BC (K20) Antibody (PACO60477)

The Crotonyl-Hist1H2BC (K20) Antibody (PAC060477) is a polyclonal antibody designed for research involving the Crotonylation of Histone H2B at lysine 20. Crotonylation is a post-translational modification of histone proteins that plays a crucial role in chromatin structure and gene expression regulation. This antibody, raised in rabbits, is highly reactive with human samples and is validated for use in various applications including Western blot and immunohistochemistry.Histone crotonylation at specific lysine residues has been linked to gene regulation, chromatin dynamics, and cellular differentiation.

The Crotonyl-Hist1H2BC (K20) Antibody specifically targets crotonylated Histone H2B at lysine 20, enabling researchers to study the role of this modification in epigenetic regulation and cellular processes. This antibody is an invaluable tool for studying chromatin modifications and their impact on gene expression, making it ideal for research in epigenetics, cancer biology, and developmental biology.

Antibody Name:Crotonyl-HIST1H2BC (K20) Antibody (PACO60477)
Antibody SKU:PACO60477
Size:50ul
Host Species:Rabbit
Tested Applications:ELISA, WB, IF, ChIP
Recommended Dilutions:ELISA:1:2000-1:10000, WB:1:100-1:1000, IF:1:10-1:100
Species Reactivity:Human
Immunogen:Peptide sequence around site of Crotonyl-Lys (20) derived from Human Histone H2B type 1-C/E/F/G/I
Form:Liquid
Storage Buffer:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:Antigen Affinity Purified
Clonality:Polyclonal
Isotype:IgG
Conjugate:Non-conjugated
Product ImageWestern Blot. Detected samples: K562 whole cell lysate, Jurkat whole cell lysate; Untreated (-) or treated (+) with 30mM Sodium crotonylate for 4h. All lanes: HIST1H2BC antibody at 1:100. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 14 kDa. Observed band size: 14 kDa.
Product ImageChromatin Immunoprecipitation Hela (106, treated with 30mM sodium crotonylate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H2BC (PACO60477) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta -Globin promoter.
Product ImageImmunofluorescence staining of Hela cells (treated with 30mM sodium crotonylate for 4h) with PACO60477 at 1:12.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Synonyms:Histone H2B type 1-C/E/F/G/I (Histone H2B.1 A) (Histone H2B.a) (H2B/a) (Histone H2B.g) (H2B/g) (Histone H2B.h) (H2B/h) (Histone H2B.k) (H2B/k) (Histone H2B.l) (H2B/l), HIST1H2BC; HIST1H2BE; HIST1H2BF; HIST1H2BG; HIST1H2BI, H2BFL; H2BFH; H2BFG; H2BFA; H2BFK
UniProt Protein Function:H2B1C: a core component of the nucleoosome. The nucleosome, a basic organizational unit of chromosomal DNA, is octrameric, consisting of two molecules each of histones H2B, H2A, H3, H4. The octamer wraps approximately 147 bp of DNA. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 6p22.1

Cellular Component: cytoplasm; extracellular space; nucleoplasm; nucleus

Molecular Function:DNA binding; protein binding

Biological Process: antibacterial humoral response; defense response to Gram-positive bacterium; innate immune response in mucosa; nucleosome assembly

NCBI Summary:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The protein has antibacterial and antifungal antimicrobial activity. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
UniProt Code:P62807
NCBI GenInfo Identifier:290457686
NCBI Gene ID:8339
NCBI Accession:P62807.4
UniProt Secondary Accession:P62807,P02278, Q3B872, Q4VB69, Q93078, Q93080,
UniProt Related Accession:P62807
Molecular Weight:13,906 Da
NCBI Full Name:Histone H2B type 1-C/E/F/G/I
NCBI Synonym Full Names:histone cluster 1, H2bg
NCBI Official Symbol:HIST1H2BG  
NCBI Official Synonym Symbols:H2B/a; H2BFA; H2B.1A; dJ221C16.8  
NCBI Protein Information:histone H2B type 1-C/E/F/G/I
UniProt Protein Name:Histone H2B type 1-C/E/F/G/I
UniProt Synonym Protein Names:Histone H2B.1 A; Histone H2B.a; H2B/a; Histone H2B.g; H2B/g; Histone H2B.h; H2B/h; Histone H2B.k; H2B/k; Histone H2B.l; H2B/l
Protein Family:Histone
UniProt Gene Name:HIST1H2BC  
UniProt Entry Name:H2B1C_HUMAN
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