The CRKII Rabbit Monoclonal Antibody (CAB9577) is a versatile tool for researchers studying CRKII, a signaling protein involved in various cellular processes such as cell migration, adhesion, and proliferation. This highly specific antibody, generated in rabbits, is suitable for use in Western blotting, immunoprecipitation, and immunofluorescence assays, allowing for the detection and analysis of CRKII in different cell types.CRKII is a key player in signal transduction pathways that control cell growth and differentiation, making it a target of interest in cancer research and developmental biology. Its role in promoting cell adhesion and migration also implicates it in diseases such as metastasis and invasion.
By utilizing the CRKII Rabbit Monoclonal Antibody, researchers can gain valuable insights into the functions of CRKII and its implications in various cellular processes and diseases.Overall, the CRKII Rabbit Monoclonal Antibody (CAB9577) is a reliable tool for investigating the role of CRKII in biological pathways and diseases, offering researchers the opportunity to advance their understanding of cellular signaling mechanisms and potentially identify new therapeutic targets for cancer and other related conditions.
Product Name:
CRK/CRKII Rabbit Monoclonal Antibody
SKU:
CAB9577
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CRK/CRKII (P46108).
This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.
Purification Method:
Affinity purification
Gene ID:
1398
Clone Number:
ARC1644
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CRK/CRK/CRKII Rabbit mAb (CAB9577) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
