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Anti-CREB1 Antibody (CAB2431)

SKU:
CAB2431
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
$167 - $503
Frequently bought together:

Description

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Anti-CREB1 Antibody (CAB2431)

The CREB1 Polyclonal Antibody (CAB2431) is a valuable tool for researchers studying CREB1, a transcription factor known for its role in gene regulation and cellular signaling. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.CREB1, also known as cAMP responsive element-binding protein 1, is involved in various cellular processes including cell proliferation, differentiation, and survival. Its dysregulation has been implicated in diseases such as cancer, neurodegenerative disorders, and metabolic conditions. By targeting CREB1 with this antibody, researchers can investigate its expression levels and activity in different cell types, providing insights into its function and potential as a therapeutic target.

With its high reactivity and specificity, the CREB1 Polyclonal Antibody is a valuable tool for studies in molecular biology, cancer research, and neuroscience. By enabling the detection and analysis of CREB1 protein, this antibody allows researchers to deepen their understanding of the pathways and mechanisms involving CREB1, ultimately contributing to advancements in biomedical research and drug development.

Antibody Name:Anti-CREB1 Antibody
Antibody SKU:CAB2431
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human CREB1 (NP_604391.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:NIH/3T3, MCF7, THP-1, Mouse brain, Mouse spleen, Mouse spinal cord, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human CREB1 (NP_604391.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MTME SGAE NQQS GDAA VTEA ENQQ MTVQ AQPQ IATL AQVS MPAA HATS SAPT VTLV QLPN GQTV QVHG VIQA AQPS VIQS PQVQ TVQS SCKD LKRL FSGT QIST IAES EDSQ ESVD SVTD
Gene ID:1385
Uniprot:P16220
Cellular Location:Nucleus
Calculated MW:25kDa/35kDa/36kDa
Observed MW:40kDa
Synonyms:CREB1, CREB, CREB-1
Background:This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms.
UniProt Protein Function:Function: This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.
UniProt Protein Details:

Subunit structure: Interacts with PPRC1. Binds DNA as a dimer. This dimer is stabilized by magnesium ions. Interacts, through the bZIP domain, with the coactivators TORC1/CRTC1, TORC2/CRTC2 and TORC3/CRTC3. When phosphorylated on Ser-133, binds CREBBP

By similarity. Interacts (phosporylated form) with TOX3. Interacts with ARRB1. Ref.8 Ref.9 Ref.11 Ref.13 Ref.14 Ref.15 Ref.16 Ref.20

Subcellular location: Nucleus Ref.12.

Post-translational modification: Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding

By similarity. Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.10 Ref.17 Ref.19Sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization. Ref.12

Involvement in Disease: Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [

MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type.

Sequence similarities: Belongs to the bZIP family.Contains 1 bZIP domain.Contains 1 KID (kinase-inducible) domain.

NCBI Summary:This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]
UniProt Code:P16220
NCBI GenInfo Identifier:117434
NCBI Gene ID:1385
NCBI Accession:P16220.2
UniProt Secondary Accession:P16220,P21934, Q9UMA7,
UniProt Related Accession:P16220,Q16366,Q16367,Q4ZG78,Q53RU9,Q53X93,Q5U0J5,Q6V963
Molecular Weight:40 kDa
NCBI Full Name:Cyclic AMP-responsive element-binding protein 1
NCBI Synonym Full Names:cAMP responsive element binding protein 1
NCBI Official Symbol:CREB1  
NCBI Official Synonym Symbols:CREB; MGC9284  
NCBI Protein Information:cyclic AMP-responsive element-binding protein 1; CREB-1; OTTHUMP00000163864; OTTHUMP00000163865; OTTHUMP00000206660; OTTHUMP00000206662; OTTHUMP00000206667; transactivator protein; active transcription factor CREB; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1
UniProt Protein Name:Cyclic AMP-responsive element-binding protein 1
Protein Family:CREB-binding protein
UniProt Gene Name:CREB1  
UniProt Entry Name:CREB1_HUMAN
Anti-CREB1 Antibody (CAB2431)Western blot analysis of extracts of various cell lines, using CREB1 antibody (CAB2431) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

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