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CREB (Phospho-Ser129) Transcription Factor Activity Assay

SKU:
TFAB00018
Product Type:
ELISA Kit
ELISA Type:
Transcription Factor Assay
Analytes:
CREB (Phospho-Ser129)
Reactivity:
Human
Mouse
Rat
€599
Frequently bought together:

Description

system_update_altDatasheet

CREB (Phospho-Ser129)Transcription Factor Activity Assay

The CREB (Phospho-Ser129) Transcription Factor Activity Assay Kit is a reliable and sensitive tool for detecting CREB transcription factor activity in cell lysates. This kit is designed for researchers studying the role of CREB in gene transcription regulation and signal transduction pathways.CREB (cAMP response element-binding protein) is a key transcription factor that plays a crucial role in the regulation of gene expression in response to various stimuli. Phosphorylation of CREB at Ser129 is known to activate its transcriptional activity, making it an important biomarker for understanding cellular signaling pathways and processes.

This assay kit offers high specificity and reproducibility, allowing for accurate quantification of CREB activity in a variety of sample types. With its user-friendly protocol and reliable results, the CREB (Phospho-Ser129) Transcription Factor Activity Assay Kit is a valuable tool for researchers in the fields of molecular biology, cell signaling, and drug discovery.

Product Name:CREB (Phospho-Ser129) Transcription Factor Activity Assay
Product Code:TFAB00018
Target:CREB (Phospho-Ser129)
Reactivity:Human, Mouse, Rat
Sample Types:Nuclear or cell lysates

The Assay Genie CREB (Phospho-Ser129) transcription factor activity assay allows for the detection and qualitative analysis of CREB (Phospho-Ser129) active transcription factors in eukaryotic nuclear or cell lysates.

Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.

Assay Time:4.5 hours
Detection Method:Colorimetric 450 nm
Size:12 x 8-Well Microstrips
Storage:4°C for 6 months
UniProt Protein Function:Function: This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.
UniProt Protein Details:

Subunit structure: Interacts with PPRC1. Binds DNA as a dimer. This dimer is stabilized by magnesium ions. Interacts, through the bZIP domain, with the coactivators TORC1/CRTC1, TORC2/CRTC2 and TORC3/CRTC3. When phosphorylated on Ser-133, binds CREBBP

By similarity. Interacts (phosporylated form) with TOX3. Interacts with ARRB1. Ref.8 Ref.9 Ref.11 Ref.13 Ref.14 Ref.15 Ref.16 Ref.20

Subcellular location: Nucleus Ref.12.

Post-translational modification: Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding

By similarity. Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.10 Ref.17 Ref.19Sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization. Ref.12

Involvement in Disease: Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [

MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type.

Sequence similarities: Belongs to the bZIP family.Contains 1 bZIP domain.Contains 1 KID (kinase-inducible) domain.

NCBI Summary:This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]
UniProt Code:P16220
NCBI GenInfo Identifier:117434
NCBI Gene ID:1385
NCBI Accession:P16220.2
UniProt Secondary Accession:P16220,P21934, Q9UMA7,
UniProt Related Accession:P16220,Q16366,Q16367,Q4ZG78,Q53RU9,Q53X93,Q5U0J5,Q6V963
Molecular Weight:40 kDa
NCBI Full Name:Cyclic AMP-responsive element-binding protein 1
NCBI Synonym Full Names:cAMP responsive element binding protein 1
NCBI Official Symbol:CREB1  
NCBI Official Synonym Symbols:CREB; MGC9284  
NCBI Protein Information:cyclic AMP-responsive element-binding protein 1; CREB-1; OTTHUMP00000163864; OTTHUMP00000163865; OTTHUMP00000206660; OTTHUMP00000206662; OTTHUMP00000206667; transactivator protein; active transcription factor CREB; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1
UniProt Protein Name:Cyclic AMP-responsive element-binding protein 1
Protein Family:CREB-binding protein
UniProt Gene Name:CREB1  
UniProt Entry Name:CREB1_HUMAN
AntibodiesELISA Kits
Anti-CREB1 Antibody [KO Validated] (CAB10826)Human CREB (Cyclic AMP Response Element Binding Protein) ELISA Kit (HUES01981)
Anti-CREB1 Antibody (CAB11063)CREB (Phospho-Ser121) Colorimetric Cell-Based ELISA Kit
Anti-CREB1 Antibody (CAB11064)CREB (Phospho-Thr100) Colorimetric Cell-Based ELISA Kit
Anti-CREB1 Antibody (CAB11989)CREB (Phospho-Ser121) Transcription Factor Activity Assay
Anti-CREB1 Antibody (CAB12311)CREB (Phospho-Ser133) Transcription Factor Activity Assay

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