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Anti-CRAT Antibody (CAB6365)

SKU:
CAB6365
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
  • Metabolism Antibodies 2 Anti-CRAT Antibody CAB6365
  • Metabolism Antibodies 2 Anti-CRAT Antibody CAB6365
€139 - €419
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Description

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Antibody Name:Anti-CRAT Antibody
Antibody SKU:CAB6365
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 357-626 of human CRAT (NP_000746.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse
Positive Samples:Mouse skeletal muscle, Mouse heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 357-626 of human CRAT (NP_000746.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:YVIE YTKK PELV RSPM VPLP MPKK LRFN ITPE IKSD IEKA KQNL SIMI QDLD ITVM VFHH FGKD FPKS EKLS PDAF IQMA LQLA YYRI YGQA CATY ESAS LRMF HLGR TDTI RSAS MDSL TFVK AMDD SSVT EHQK VELL RKAV QAHR GYTD RAIR GEAF DRHL LGLK LQAI EDLV SMPD IFMD TSYA IAMH FHLS TSQV PAKT DCVM FFGP VVPD GYGV CYNP MEAH INFS LSAY NSCA ETNA ARLA HYLE KALL DMRA LLQS HPRA KL
Gene ID:1384
Uniprot:P43155
Cellular Location:Endoplasmic reticulum, Matrix side, Mitochondrion, Mitochondrion inner membrane, Peripheral membrane protein, Peroxisome
Calculated MW:61kDa/68kDa/70kDa
Observed MW:60kDa
Synonyms:CRAT, CAT, CAT1
Background:This gene encodes carnitine acetyltransferase (CRAT), which is a key enzyme in the metabolic pathway in mitochondria, peroxisomes and endoplasmic reticulum. CRAT catalyzes the reversible transfer of acyl groups from an acyl-CoA thioester to carnitine and regulates the ratio of acylCoA/CoA in the subcellular compartments. Two transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:CRAT: Carnitine acetylase is specific for short chain fatty acids. Carnitine acetylase seems to affect the flux through the pyruvate dehydrogenase complex. It may be involved as well in the transport of acetyl-CoA into mitochondria. Belongs to the carnitine/choline acetyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 2.3.1.7; Mitochondrial; Acetyltransferase

Chromosomal Location of Human Ortholog: 9q34.1

Cellular Component: peroxisomal matrix; mitochondrion; endoplasmic reticulum; mitochondrial inner membrane; peroxisome

Molecular Function:carnitine O-acetyltransferase activity; receptor binding

Biological Process: carnitine metabolic process, CoA-linked; fatty acid beta-oxidation using acyl-CoA oxidase; transport; cellular lipid metabolic process

NCBI Summary:This gene encodes carnitine acetyltransferase (CRAT), which is a key enzyme in the metabolic pathway in mitochondria, peroxisomes and endoplasmic reticulum. CRAT catalyzes the reversible transfer of acyl groups from an acyl-CoA thioester to carnitine and regulates the ratio of acylCoA/CoA in the subcellular compartments. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
UniProt Code:P43155
NCBI GenInfo Identifier:215274265
NCBI Gene ID:1384
NCBI Accession:P43155.5
UniProt Secondary Accession:P43155,Q5T952, Q9BW16,
UniProt Related Accession:P43155
Molecular Weight:626
NCBI Full Name:Carnitine O-acetyltransferase
NCBI Synonym Full Names:carnitine O-acetyltransferase
NCBI Official Symbol:CRAT  
NCBI Official Synonym Symbols:CAT1  
NCBI Protein Information:carnitine O-acetyltransferase; CAT; carnitine acetylase; carnitine acetyltransferase
UniProt Protein Name:Carnitine O-acetyltransferase
UniProt Synonym Protein Names:Carnitine acetyltransferase; CAT; CrAT
Protein Family:Carnitine O-acetyltransferase
UniProt Gene Name:CRAT  
UniProt Entry Name:CACP_HUMAN
Anti-CRAT Antibody (CAB6365)Western blot analysis of extracts of various cell lines, using CRAT antibody (CAB6365) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
Anti-CRAT Antibody (CAB6365)Immunofluorescence analysis of HeLa cells using CRAT antibody (CAB6365). Blue: DAPI for nuclear staining.

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