Anti-CR1 Antibody (CAB10059)

Antibody Name:	Anti-CR1 Antibody
Antibody SKU:	CAB10059
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 420-550 of human CR1 (NP_000642.3).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human
Positive Samples:	U-937

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 420-550 of human CR1 (NP_000642.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FCPS PPVI PNGR HTGK PLEV FPFG KTVN YTCD PHPD RGTS FDLI GEST IRCT SDPQ GNGV WSSP APRC GILG HCQA PDHF LFAK LKTQ TNAS DFPI GTSL KYEC RPEY YGRP FSIT CLDN LVWS SPKD VCK
Gene ID:	1378
Uniprot:	P17927
Cellular Location:	Membrane, Single-pass type I membrane protein
Calculated MW:	223kDa
Observed MW:	270kDa

Synonyms:

CR1, C3BR, C4BR, CD35, KN

Background:

This gene is a member of the receptors of complement activation (RCA) family and is located in the 'cluster RCA' region of chromosome 1. The gene encodes a monomeric single-pass type I membrane glycoprotein found on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. The Knops blood group system is a system of antigens located on this protein. The protein mediates cellular binding to particles and immune complexes that have activated complement. Decreases in expression of this protein and/or mutations in its gene have been associated with gallbladder carcinomas, mesangiocapillary glomerulonephritis, systemic lupus erythematosus and sarcoidosis. Mutations in this gene have also been associated with a reduction in Plasmodium falciparum rosetting, conferring protection against severe malaria. Alternate allele-specific splice variants, encoding different isoforms, have been characterized. Additional allele specific isoforms, including a secreted form, have been described but have not been fully characterized.

UniProt Protein Function:	CR1: Mediates cellular binding of particles and immune complexes that have activated complement. Belongs to the receptors of complement activation (RCA) family.
UniProt Protein Details:	Protein type:Membrane protein, integral; Receptor, misc. Chromosomal Location of Human Ortholog: 1q32 Cellular Component: cell surface; integral to plasma membrane; plasma membrane Molecular Function:complement component C3b receptor activity; complement component C4b binding; complement component C4b receptor activity; complement component C3b binding Biological Process: negative regulation of complement activation, alternative pathway; complement receptor mediated signaling pathway; negative regulation of complement activation, classical pathway; regulation of complement activation; innate immune response; complement activation, classical pathway Disease: Knops Blood Group System; Malaria, Susceptibility To
NCBI Summary:	This gene is a member of the receptors of complement activation (RCA) family and is located in the 'cluster RCA' region of chromosome 1. The gene encodes a monomeric single-pass type I membrane glycoprotein found on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. The Knops blood group system is a system of antigens located on this protein. The protein mediates cellular binding to particles and immune complexes that have activated complement. Decreases in expression of this protein and/or mutations in its gene have been associated with gallbladder carcinomas, mesangiocapillary glomerulonephritis, systemic lupus erythematosus and sarcoidosis. Mutations in this gene have also been associated with a reduction in Plasmodium falciparum rosetting, conferring protection against severe malaria. Alternate allele-specific splice variants, encoding different isoforms, have been characterized. Additional allele specific isoforms, including a secreted form, have been described but have not been fully characterized. [provided by RefSeq, Jul 2008]
UniProt Code:	P17927
NCBI GenInfo Identifier:	86793036
NCBI Gene ID:	1378
NCBI Accession:	NP_000564.2
UniProt Secondary Accession:	P17927,Q16744, Q16745, Q5SR43, Q5SR45, Q9UQV2,
UniProt Related Accession:	P17927
Molecular Weight:	223,663 Da
NCBI Full Name:	complement receptor type 1 isoform F
NCBI Synonym Full Names:	complement component (3b/4b) receptor 1 (Knops blood group)
NCBI Official Symbol:	CR1
NCBI Official Synonym Symbols:	KN; C3BR; C4BR; CD35
NCBI Protein Information:	complement receptor type 1
UniProt Protein Name:	Complement receptor type 1
UniProt Synonym Protein Names:	C3b/C4b receptor; CD_antigen: CD35
Protein Family:	Complement receptor
UniProt Gene Name:	CR1
UniProt Entry Name:	CR1_HUMAN

	Western blot analysis of extracts of U-937 cells, using CR1 antibody (CAB10059) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunofluorescence analysis of THP-1 cells using CR1 antibody (CAB10059) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Human CD35 Monoclonal Antibody [E11](AGEL0257)	Human C3BR / CD35 / Complement Receptor type 1 ELISA Kit
Human CD35 Monoclonal Antibody (Biotin Conjugated) [E11](AGEL0258)	Human CR1 (Complement Receptor type 1) CLIA Kit (HUES01153)
Human CD35 Monoclonal Antibody (FITC Conjugated) [E11](AGEL1010)
Human CD35 Monoclonal Antibody (PE Conjugated) [E11](AGEL1011)
Human CD35 Monoclonal Antibody (APC Conjugated) [E11](AGEL1012)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)