The Anti-CPT1A Antibody (CAB20746) offered by AssayGenie is a high-quality polyclonal antibody designed for research involving Carnitine Palmitoyltransferase 1A (CPT1A), an enzyme involved in fatty acid metabolism. This antibody, raised in rabbits, exhibits strong reactivity with human samples and has been validated for use in Western blot applications.CPT1A plays a crucial role in the transportation of long-chain fatty acids into the mitochondria for beta-oxidation, making it essential for energy production in various cell types.
Dysregulation of CPT1A has been implicated in metabolic disorders, such as obesity and diabetes, as well as certain types of cancer.By targeting the CPT1A protein, researchers can effectively study its function and regulation in normal and disease states. This antibody is a valuable tool for investigations in metabolism, energy balance, and related diseases, providing insights that may lead to potential therapeutic strategies in the future.
Product Name:
CPT1A Monoclonal Antibody
SKU:
CAB20746
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 520-720 of human CPT1A (NP_001867.2).
The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
1374
Clone Number:
ARC51171
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from MCF7 cells, using CPT1A Rabbit mAb (CAB20746) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
