CPSF4 Rabbit Polyclonal Antibody (CAB10284)
- SKU:
- CAB10284
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
Product Name: | CPSF4 Rabbit Polyclonal Antibody |
SKU: | CAB10284 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-244 of human CPSF4 (NP_001075028.1). |
Sequence: | MQEI IASV DHIK FDLE IAVE QQLG AQPL PFPG MDKS GAAV CEFF LKAA CGKG GMCP FRHI SGEK TVVC KHWL RGLC KKGD QCEF LHEY DMTK MPEC YFYS KFGE CSNK ECPF LHID PESK IKDC PWYD RGFC KHGP LCRH RHTR RVIC VNYL VGFC PEGP SCKF MHPR FELP MGTT EQPP LPQQ TQPP AKQR TPQV IGVM QSQN SSAG NRGP RPLE QVTC YKCG EKGH YANR CTKG HLAF LSGQ |
Tested Applications: | WB ELISA |
Recommended Dilution: | WB,1:1000 - 1:2000 |
Synonyms: | NAR; NEB1; NEB-1; CPSF30; CPSF4 |
Positive Sample: | HeLa,Jurkat,22Rv1 |
Conjugate: | Unconjugated |
Cellular Localization: | Nucleus. |
Calculated MW: | 30kDa |
Observed MW: | 30kDa |
Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs. Multiple alternatively spliced transcript variants that encode different isoforms have been described for this gene.
Purification Method: | Affinity purification |
Gene ID: | 10898 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |