The CPSF3L Polyclonal Antibody (CAB6566) is a valuable tool for researchers studying CPSF3L, a key protein involved in mRNA processing and gene expression regulation. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for Western blot applications. By targeting the CPSF3L protein, this antibody allows for accurate detection and analysis in various cell types, making it ideal for studies in molecular biology and gene regulation.CPSF3L is a component of the cleavage and polyadenylation specificity factor (CPSF) complex, which plays a crucial role in post-transcriptional mRNA processing.
Dysregulation of CPSF3L has been linked to defects in mRNA processing and gene expression, making it a potential target for research into diseases like cancer, neurological disorders, and developmental abnormalities. By gaining a better understanding of CPSF3L function, researchers can uncover new insights into gene regulation mechanisms and potentially develop novel therapeutic approaches.
Product Name:
CPSF3L Rabbit Polyclonal Antibody
SKU:
CAB6566
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 331-600 of human CPSF3L (NP_060341.2).
WB,1:500 - 1:2000 IF/ICC,1:10 - 1:100 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
RC68; INT11; RC-68; CPSF3L; CPSF73L
Positive Sample:
HeLa,SKOV3,MCF7,Mouse spleen,Rat liver
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
68kDa
Observed MW:
68kDa
The Integrator complex contains at least 12 subunits and associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates the 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690). INTS11, or CPSF3L, is the catalytic subunit of the Integrator complex (Baillat et al., 2005 [PubMed 16239144]).
Purification Method:
Affinity purification
Gene ID:
54973
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using CPSF3L Rabbit pAb (CAB6566) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
