The CPSF1 Polyclonal Antibody (CAB17144) is specifically designed for research involving CPSF1, a key protein complex involved in mRNA processing and regulation of gene expression. This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in Western blot and immunofluorescence applications.CPSF1 is a critical component of the Cleavage and Polyadenylation Specificity Factor (CPSF) complex, which plays a crucial role in the cleavage and polyadenylation of mRNA molecules. Dysregulation of CPSF1 has been linked to various diseases, including cancer and neurological disorders, making it a valuable target for further research.
By using the CPSF1 Polyclonal Antibody, researchers can detect and analyze CPSF1 protein levels in different cell types, providing valuable insights into its role in gene expression regulation. This antibody is an essential tool for studies in molecular biology, genetics, and biomedicine, offering a deeper understanding of the mechanisms underlying mRNA processing and its impact on human health.
Product Name:
CPSF1 Rabbit Polyclonal Antibody
SKU:
CAB17144
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 700-950 of human CPSF1 (NP_037423.2).
Cleavage and polyadenylation specificity factor (CPSF) is a multisubunit complex that plays a central role in 3-prime processing of pre-mRNAs. CPSF recognizes the AAUAAA signal in the pre-mRNA and interacts with other proteins to facilitate both RNA cleavage and poly(A) synthesis. CPSF1 is the largest subunit of the CPSF complex (Murthy and Manley, 1995 [PubMed 7590244]).
Purification Method:
Affinity purification
Gene ID:
29894
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CPSF1 Rabbit pAb (CAB17144) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
