The CPNE7 Polyclonal Antibody (PAC061169) is a valuable research tool designed for studying the CPNE7 protein, a member of the copine family that plays a role in intracellular membrane trafficking and calcium-dependent signaling pathways. This antibody, derived from rabbit immunization, exhibits high reactivity with human samples and has been validated for use in Western blot applications.The CPNE7 antibody allows for the specific detection and analysis of the CPNE7 protein in various cell types, making it an essential tool for researchers interested in understanding the molecular mechanisms underlying membrane dynamics and cellular signaling.
Its utility extends to studies in cell biology, neurobiology, and cancer research, where CPNE7's involvement in cellular processes offers insights into disease pathogenesis and potential therapeutic targets.By targeting CPNE7, researchers can further explore its functions in cell physiology and disease progression, paving the way for innovative treatments and diagnostic tools in a variety of pathological conditions. The CPNE7 Polyclonal Antibody is a valuable asset for advancing scientific knowledge and driving discoveries in the fields of molecular biology and biomedicine.
IHC image of PACO61169 diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot. Positive WB detected in: U87 whole cell lysate, SH-SY5Y whole cell lysate, HepG2 whole cell lysate, Mouse brain tissue, Rat heart tissue. All lanes: CPNE7 antibody at 3.6µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 71, 62 kDa. Observed band size: 62 kDa.
Immunofluorescence staining of A549 cells with PACO61169 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Calcium-dependent phospholipid-binding protein that may play a role in calcium-mediated intracellular processes.
Synonyms:
Copine-7 (Copine VII), CPNE7
UniProt Protein Function:
CPNE7: May function in membrane trafficking. Exhibits calcium- dependent phospholipid binding properties. Belongs to the copine family. 2 isoforms of the human protein are produced by alternative splicing.Chromosomal Location of Human Ortholog: 16q24.3Molecular Function: transporter activityBiological Process: transport; lipid metabolic process
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the copine family, which is composed of calcium-dependent membrane-binding proteins. The gene product contains two N-terminal C2 domains and one von Willebrand factor A domain. The encoded protein may be involved in membrane trafficking. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]
