CPNE7 Antibody (PACO61169)
- SKU:
- PACO61169
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| Antibody Name: | CPNE7 Antibody (PACO61169) |
| Antibody SKU: | PACO61169 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Recombinant Human Copine-7 protein (254-393AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO61169 diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Western Blot. Positive WB detected in: U87 whole cell lysate, SH-SY5Y whole cell lysate, HepG2 whole cell lysate, Mouse brain tissue, Rat heart tissue. All lanes: CPNE7 antibody at 3.6µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 71, 62 kDa. Observed band size: 62 kDa. |
 | Immunofluorescence staining of A549 cells with PACO61169 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Calcium-dependent phospholipid-binding protein that may play a role in calcium-mediated intracellular processes. |
| Synonyms: | Copine-7 (Copine VII), CPNE7 |
| UniProt Protein Function: | CPNE7: May function in membrane trafficking. Exhibits calcium- dependent phospholipid binding properties. Belongs to the copine family. 2 isoforms of the human protein are produced by alternative splicing.Chromosomal Location of Human Ortholog: 16q24.3Molecular Function: transporter activityBiological Process: transport; lipid metabolic process |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a member of the copine family, which is composed of calcium-dependent membrane-binding proteins. The gene product contains two N-terminal C2 domains and one von Willebrand factor A domain. The encoded protein may be involved in membrane trafficking. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008] |
| UniProt Code: | Q9UBL6 |
| NCBI GenInfo Identifier: | 10719954 |
| NCBI Gene ID: | 27132 |
| NCBI Accession: | Q9UBL6.1 |
| UniProt Secondary Accession: | Q9UBL6 |
| UniProt Related Accession: | Q9UBL6 |
| Molecular Weight: | 61,933 Da |
| NCBI Full Name: | Copine-7 |
| NCBI Synonym Full Names: | copine VII |
| NCBI Official Symbol: | CPNE7Â Â |
| NCBI Official Synonym Symbols: | |
| NCBI Protein Information: | copine-7; copine 7 |
| UniProt Protein Name: | Copine-7 |
| UniProt Synonym Protein Names: | Copine VII |
| Protein Family: | Copine |
| UniProt Gene Name: | CPNE7Â Â |
| UniProt Entry Name: | CPNE7_HUMAN |