CPEB4 Antibody (PACO35486)
- SKU:
- PACO35486
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
CPEB4 Antibody (PACO35486)
The CPEB4 Polyclonal Antibody (PAC035486) is a valuable tool for researchers studying CPEB4, a key player in RNA regulation and associated with cancer progression and metastasis. This antibody, produced in rabbits, exhibits high reactivity towards human samples and is suitable for Western blot applications. By specifically binding to the CPEB4 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential component of studies focusing on RNA regulation, cancer biology, and metastasis mechanisms.CPEB4, also known as cytoplasmic polyadenylation element-binding protein 4, is a critical regulator of mRNA translation and stability, influencing key cellular processes such as cell proliferation, differentiation, and migration.
Its dysregulation has been linked to various cancers, making it a promising target for therapeutic interventions aimed at controlling tumor growth and metastatic spread. Investigating the role of CPEB4 in these contexts is crucial for advancing our understanding of cancer biology and developing targeted treatment strategies.
| Antibody Name: | CPEB4 Antibody (PACO35486) |
| Antibody SKU: | PACO35486 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Cytoplasmic polyadenylation element-binding protein 4 protein (244-515AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot. All lanes: CPEB4 antibody at 2µg/ml. Lane 1: Hela whole cell lysate. Lane 2: 293T whole cell lysate. Lane 3: U87 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 81, 79, 37 kDa. Observed band size: 81 kDa. |
 | Immunofluorescence staining of Hela cells with PACO35486 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Sequence-specific RNA-binding protein that binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3'-UTR. RNA binding results in a clear conformational change analogous to the Venus fly trap mechanism. Regulates activation of unfolded protein response (UPR) in the process of adaptation to ER stress in liver, by maintaining translation of CPE-regulated mRNAs in conditions in which global protein synthesis is inhibited (By similarity). Required for cell cycle progression, specifically for cytokinesis and chromosomal segregation. Plays a role as an oncogene promoting tumor growth and progression by positively regulating translation of t-plasminogen activator/PLAT. Stimulates proliferation of melanocytes. In contrast to CPEB1 and CPEB3, does not play role in synaptic plasticity, learning and memory (By similarity). |
| Synonyms: | Cytoplasmic polyadenylation element-binding protein 4 (CPE-BP4) (CPE-binding protein 4) (hCPEB-4), CPEB4, KIAA1673 |
| UniProt Protein Function: | CPEB4: Belongs to the RRM CPEB family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:RNA-binding Chromosomal Location of Human Ortholog: 5q21 Cellular Component: cytoplasm; dendrite; endoplasmic reticulum; neuron projection; nucleus; postsynaptic density; synapse Molecular Function:mRNA 3'-UTR binding; nucleotide binding; protein binding; ribosome binding; translation factor activity, nucleic acid binding; translation repressor activity, nucleic acid binding Biological Process: cellular response to glucose starvation; ionotropic glutamate receptor signaling pathway; negative regulation of neuron apoptosis; translation |
| UniProt Code: | Q17RY0 |
| NCBI GenInfo Identifier: | 815891022 |
| NCBI Gene ID: | 80315 |
| NCBI Accession: | NP_001295118.1 |
| UniProt Secondary Accession: | Q17RY0,Q7Z310, Q8N405, Q9C0J0, B7ZLQ7, |
| UniProt Related Accession: | Q17RY0 |
| Molecular Weight: | 36,212 Da |
| NCBI Full Name: | cytoplasmic polyadenylation element-binding protein 4 isoform b |
| NCBI Synonym Full Names: | cytoplasmic polyadenylation element binding protein 4 |
| NCBI Official Symbol: | CPEB4Â Â |
| NCBI Official Synonym Symbols: | CPE-BP4; hCPEB-4Â Â |
| NCBI Protein Information: | cytoplasmic polyadenylation element-binding protein 4 |
| UniProt Protein Name: | Cytoplasmic polyadenylation element-binding protein 4 |
| Protein Family: | Cytoplasmic polyadenylation element-binding protein |
| UniProt Gene Name: | CPEB4Â Â |
| UniProt Entry Name: | CPEB4_HUMAN |
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