The COX7A2L Polyclonal Antibody (CAB8298) is specifically designed for research involving COX7A2L, a protein found in the mitochondria that plays a crucial role in cellular respiration. This antibody, produced in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. By binding to the COX7A2L protein, researchers can easily detect and analyze its expression in various cell types, making it an essential tool for studies in mitochondrial function and metabolism research.COX7A2L is a subunit of cytochrome c oxidase, a key enzyme in the electron transport chain that generates ATP, the energy currency of the cell. Dysregulation of COX7A2L has been implicated in various metabolic disorders and diseases, making it a promising target for therapeutic interventions.
Understanding the role of COX7A2L in cellular respiration is crucial for developing treatments for conditions related to mitochondrial dysfunction and metabolic disorders.Overall, the COX7A2L Polyclonal Antibody (CAB8298) is a valuable tool for researchers investigating mitochondrial function, metabolism, and the role of COX7A2L in various health conditions. Its high specificity and sensitivity make it a reliable option for Western blot analysis and other experimental techniques in the field of cellular respiration and energetics research.
Product Name:
COX7A2L Rabbit Polyclonal Antibody
SKU:
CAB8298
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-114 of human COX7A2L (NP_004709.2).
Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein similar to polypeptides 1 and 2 of subunit VIIa in the C-terminal region, and also highly similar to the mouse Sig81 protein sequence. This gene is expressed in all tissues, and upregulated in a breast cancer cell line after estrogen treatment. It is possible that this gene represents a regulatory subunit of COX and mediates the higher level of energy production in target cells by estrogen. Several transcript variants, some protein-coding and others non-protein coding, have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
9167
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using COX7A2L antibody (CAB8298) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
