The Cox7a2l Polyclonal Antibody (AB10025R) is an essential tool for conducting research on Cox7a2l, a protein involved in mitochondrial function and energy metabolism regulation. This antibody, produced in rabbits, specifically recognizes the human Cox7a2l protein and has been validated for use in Western blot applications. By binding to Cox7a2l, this antibody allows for accurate detection and analysis of the protein in various cell types, making it a valuable asset for studies in bioenergetics, metabolism, and mitochondrial dysfunction.Cox7a2l is a crucial component of the mitochondrial respiratory chain, playing a key role in electron transport and oxidative phosphorylation processes.
Dysregulation of Cox7a2l has been linked to various metabolic disorders, including obesity, diabetes, and mitochondrial diseases. Investigating the function and expression of Cox7a2l can provide insights into the mechanisms underlying these conditions and guide the development of potential therapeutic interventions targeting mitochondrial dysfunction.Overall, the Cox7a2l Polyclonal Antibody serves as a reliable tool for researchers exploring the role of Cox7a2l in metabolic pathways and mitochondrial function, offering valuable insights into the molecular mechanisms that govern energy production and cellular homeostasis.
Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein similar to polypeptides 1 and 2 of subunit VIIa in the C-terminal region, and also highly similar to the mouse Sig81 protein sequence. This gene is expressed in all tissues, and upregulated in a breast cancer cell line after estrogen treatment. It is possible that this gene represents a regulatory subunit of COX and mediates the higher level of energy production in target cells by estrogen. Several transcript variants, some protein-coding and others non-protein coding, have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
9167
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type(WT) and COX7A2L knockdown (KD) HeLa cells, using [KD Validated] COX7A2L Rabbit pAb (CAB23681) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
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