The COX5A Polyclonal Antibody is a high-quality research tool designed for studying the COX5A protein, a key subunit of cytochrome c oxidase, an essential enzyme in the respiratory chain. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the COX5A protein, researchers can accurately detect and analyze its expression in various cell types, making it a valuable tool for studies in mitochondrial biology and respiratory chain function.COX5A is known to play a crucial role in mitochondrial energy production and cellular respiration. Mutations or dysregulation of this protein have been linked to various mitochondrial diseases and disorders.
Research on COX5A is essential for understanding the mechanisms underlying mitochondrial dysfunction and could potentially lead to the development of targeted therapies for mitochondrial disorders and metabolic diseases.Overall, the COX5A Polyclonal Antibody offers researchers a reliable and effective tool for investigating the function and regulation of the COX5A protein in cellular energy metabolism and mitochondrial function. Its specificity and sensitivity make it an ideal choice for studies in bioenergetics, mitochondrial biology, and related research areas.
Product Name:
COX5A Polyclonal Antibody
SKU:
CAB24500
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human COX5A (NP_004246.2).
COX5A; COX; COX-VA; VA; cytochrome c oxidase subunit 5A; 5A
Positive Sample:
HeLa,Mouse brain,Mouse heart,Rat brain
Conjugate:
Unconjugated
Cellular Localization:
Mitochondrion inner membrane.
Calculated MW:
16kDa
Observed MW:
15kDa
Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Va of the human mitochondrial respiratory chain enzyme. A pseudogene COX5AP1 has been found in chromosome 14q22.
Purification Method:
Affinity purification
Gene ID:
9377
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using [KO Validated] COX5A Rabbit pAb (CAB24500) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
![Western blot analysis of various lysates, using [KO Validated] COX5A Rabbit pAb (CAB24500) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/1280x1280/products/224342/583604/A24500_N29296-4_WB_01__06975.1701346913.jpg?c=2&imbypass=on "Western blot analysis of various lysates, using [KO Validated] COX5A Rabbit pAb (CAB24500) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
