The COX4I1 Polyclonal Antibody (CAB6564) is a valuable tool for researchers studying mitochondrial function and cellular respiration. This antibody, generated in rabbits, is highly specific to the COX4I1 protein and is validated for use in applications such as Western blot and immunofluorescence.COX4I1 is a subunit of cytochrome c oxidase (complex IV) in the mitochondrial electron transport chain, playing a crucial role in the oxidative phosphorylation process.
Aberrant expression or mutations in COX4I1 have been linked to various diseases, including metabolic disorders and cancer. By using the COX4I1 Polyclonal Antibody, researchers can accurately detect and measure COX4I1 levels in different cell types and tissues, providing insights into mitochondrial function and potential therapeutic targets for related diseases.
Product Name:
COX IV Rabbit Polyclonal Antibody
SKU:
CAB6564
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 23-98 of human COX IV (NP_001852.1).
WB,1:1000 - 1:5000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
COX4; COXIV; COX4-1; COXIV-1; MC4DN16; COX IV-1; COX IV
Positive Sample:
293T,Mouse liver,Rat brain
Conjugate:
Unconjugated
Cellular Localization:
Mitochondrion inner membrane.
Calculated MW:
20kDa
Observed MW:
17kDa
Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes 13 and 14. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
1327
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using COX IV Rabbit pAb (CAB6564) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
