The Cox11 Polyclonal Antibody (CAB16290) is a valuable tool for research involving the Cox11 protein, which plays a crucial role in the assembly of cytochrome c oxidase, an essential component of the mitochondrial respiratory chain. This antibody, produced in rabbits, is highly specific to human samples and is verified for use in Western blot applications. By binding to the Cox11 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for studies in mitochondrial biology and related diseases.The Cox11 protein is essential for the proper functioning of the mitochondrial electron transport chain and oxidative phosphorylation, processes that are vital for cellular energy production.
Dysregulation of Cox11 has been linked to various disorders, including mitochondrial diseases and neurodegenerative conditions. Research on Cox11 is crucial for understanding the mechanisms underlying these diseases and developing potential therapeutic interventions targeting mitochondrial dysfunction.Overall, the Cox11 Polyclonal Antibody is a valuable tool for scientists studying mitochondrial biology, energy metabolism, and related diseases. Its high specificity and reliability make it an excellent choice for accurate and reproducible experimental results in the field of molecular biology and biochemistry.
Product Name:
COX11 Rabbit Polyclonal Antibody
SKU:
CAB16290
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 140-240 of human COX11 (NP_004366.1).
Sequence:
ENMV PVKD RIIK ISFN ADVH ASLQ WNFR PQQT EIYV VPGE TALA FYRA KNPT DKPV IGIS TYNI VPFE AGQY FNKI QCFC FEEQ RLNP QEEV DMPV FFYI D
Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein which is not a structural subunit, but may be a heme A biosynthetic enzyme involved in COX formation, according to the yeast mutant studies. However, the studies in Rhodobacter sphaeroides suggest that this gene is not required for heme A biosynthesis, but required for stable formation of the Cu(B) and magnesium centers of COX. This human protein is predicted to contain a transmembrane domain localized in the mitochondrial inner membrane. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene has been found on chromosome 6.
Purification Method:
Affinity purification
Gene ID:
1353
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using COX11 Rabbit pAb (CAB16290) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
