The Cox10 Polyclonal Antibody (CAB7547) is a valuable tool for researchers studying the Cox10 protein, an essential component of the mitochondrial respiratory chain. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications.Cox10 is involved in the biosynthesis of heme, a crucial component of various proteins such as cytochromes and catalases. Dysfunction of Cox10 can lead to mitochondrial disorders, impacting cellular energy production and overall health. By targeting the Cox10 protein, researchers can investigate its role in mitochondrial function and its potential implications in disease states.
The Cox10 Polyclonal Antibody enables the detection and analysis of Cox10 protein levels in a variety of cell types, making it a versatile tool for researchers in the fields of biochemistry, cell biology, and mitochondrial research. By studying the function and regulation of Cox10, scientists can gain insights into mitochondrial biology and potentially identify new therapeutic targets for mitochondrial diseases.
Product Name:
COX10 Rabbit Polyclonal Antibody
SKU:
CAB7547
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human COX10 (NP_001294.2).
Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes heme A:farnesyltransferase, which is not a structural subunit but required for the expression of functional COX and functions in the maturation of the heme A prosthetic group of COX. This protein is predicted to contain 7-9 transmembrane domains localized in the mitochondrial inner membrane. A gene mutation, which results in the substitution of a lysine for an asparagine (N204K), is identified to be responsible for cytochrome c oxidase deficiency. In addition, this gene is disrupted in patients with CMT1A (Charcot-Marie-Tooth type 1A) duplication and with HNPP (hereditary neuropathy with liability to pressure palsies) deletion.
Purification Method:
Affinity purification
Gene ID:
1352
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of mouse esophagus, using COX10 Antibody (CAB7547) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
